The Dietary Supplement Protandim® Decreases Plasma Osteopontin and Improves Markers of Oxidative Stress in Muscular Dystrophy Mdx Mice

Joe McCord and Brian Tseng published this.

 

The Dietary Supplement Protandim® Decreases Plasma Osteopontin and Improves Markers of Oxidative Stress in Muscular Dystrophy Mdx Mice

http://informahealthcare.com/eprint/iitYPNajYiq4mNmPt6Mg/full?token...

Muhammad Muddasir Qureshi, MD, MPH Warren C. McClure, MS Nicole L. Arevalo, MA Rick E. Rabon, BA Benjamin Mohr‌ Swapan K. Bose, BS, BPharm Joe M. McCord, PhD Brian S. Tseng, MD, PhD
Address correspondence to: Brian S. Tseng, MD, PhD, Department of Neurology, Division of Child Neurology, Massachusetts General Hospital, Harvard Medical School, 55 Fruit St ACC 708, Boston, MA 02114 (E-mail: bstseng@partners.org).



ABSTRACT.

Therapeutic options for Duchenne muscular dystrophy (DMD), the most common and lethal neuromuscular disorder in children, remain elusive. Oxidative damage is implicated as a pertinent factor involved in its pathogenesis. Protandim® is an over-the-counter supplement with the ability to induce antioxidant enzymes. In this study we investigated whether Protandim® provided benefit using surrogate markers and functional measures in the dystrophin-deficient (mdx) mouse model of DMD. Male 3-week-old mdx mice were randomized into two treatment groups: control (receiving standard rodent chow) and Protandim®-supplemented standard rodent chow. The diets were continued for 6-week and 6-month studies. The endpoints included the oxidative stress marker thiobarbituric acid-reactive substances (TBARS), plasma osteopontin (OPN), plasma paraoxonase (PON1) activity, H&E histology, gadolinium-enhanced magnetic resonance imaging (MRI) of leg muscle and motor functional measurements. The Protandim® chow diet in mdx mice for 6 months was safe and well tolerated. After 6 months of Protandim®, a 48% average decrease in plasma TBARS was seen; 0.92 nmol/mg protein in controls versus 0.48 nmol/mg protein in the Protandim® group (p = .006). At 6 months, plasma OPN was decreased by 57% (p = .001) in the Protandim®-treated mice. Protandim® increased the plasma antioxidant enzyme PON1 activity by 35% (p = .018). After 6 months, the mdx mice with Protandim® showed 38% less MRI signal abnormality (p = .07) than mice on control diet. In this 6-month mdx mouse study, Protandim® did not significantly alter motor function nor histological criteria.

 

 

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Somewhat confused here, the article states Plasma-T Bars improve with Protandim (don't know what a Plasma-T Bar is) but the ending sentence states Protandim did not sig alter motor function nor histological criteria. Not sure what this article says. Help! :)
I think what it is saying is that the muscle breakdown process showed signs of being slowed down, however it isnt stopped so there is no gain in strength and the muscle breakdown is still ongoing, just at a slower rate. Thats my take anyway!
I read the report - on balance, protandim seems beneficial - the final paragraph:

Despite these limitations, the study provides evidence of reduced oxidative damage, and perhaps of a reduced profibrotic state, with the use of Protandim® in mdx mice. The use of Protandim® did not cause any untoward side effects and overall the compound appeared safe. Pharmacokinetic studies and long-term trials of Protandim® in mdx mice and humans with DMD are required to determine its impact on DMD disease progression and survival.
Additionally this was only a 6 mth study, it would be interesting to see what happens in a longer term study. Also interesting to see what would happen in humans...so many boys are taking Protandim, it would be good to collect some controlled data...not sure how expensive this can be though.

Jonathan said:
I think what it is saying is that the muscle breakdown process showed signs of being slowed down, however it isnt stopped so there is no gain in strength and the muscle breakdown is still ongoing, just at a slower rate. Thats my take anyway!
Here are two older papers on Protandim.
Attachments:

http://jeremybernsdorff.com/blog/uploaded/files/PDFs/Protandim_DMD_...

 

Here is the link to the parent observational study of Protandim.  It was done in Europe in 2009.  It looks there are positive benefits.  Kind of long but you can scroll down.

If anyone is interested in trying Protandim and getting the discounted rate of $40 on autoship, please go to my link.  I am a Duchenne mom who recently began selling Protandim to spread the word.  My husband and I are also on it as well.  I noticed that with a lot of other Duchenne parent's supplement lists, that they were on it too.  Light bulb moment!  We can all take advantage of reduced oxidative stress!  Thanks!  www.mylifevantage.com/tgriffin

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